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Features of RSpak DE Series (4) (Reproducibility between Production Lots)

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The production of packing materials for RSpak DE-413 is done in single stage, and, that of ODS columns is done in two stages, production of base material and bonding of C18 group. The simple production process of DE series ensures good reproducibility between production lots.

Lot ALot BLot C
No.Rt
(min)
Peak
area
UV x sec
Peak
area
%
No.Rt
(min)
Peak
area
UV x sec
Peak
area
%
No.Rt
(min)
Peak
area
UV x sec
Peak
area
%
1 2.50 36600 20.1 1 2.50 37600 20.1 1 2.48 36400 20.2
2 3.15 32200 17.7 2 3.152 33200 17.7 2 3.12 31900 17.7
3 4.88 48600 26.7 3 4.89 50100 26.7 3 4.85 48100 26.6
4 7.96 64800 35.6 4 8.00 66700 35.6 4 7.92 64300 35.6


Sample : 5 μL
1. 0.2 % Methyl ethyl ketone, 2-Butanone
2. 0.2 % Diethyl ketone, 3-Pentanone
3. 0.4 % Di-n-propyl ketone, 4-Heptanone
4. 0.6 % Di-n-butyl ketone, 5-Nonanone


Column       : Shodex RSpak DE-413 (4.6 mm I.D. x 150 mm)
Eluent       : CH3CN/H2O=50/50
Flow rate    : 1.0 mL/min
Detector     : UV (262 nm)
Column temp. : 40 °C

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