Features of RSpak DE Series (5) (Selection of HPLC System)

To realize the best performance of RSpak DE-413 column, we recommend to use the following HPLC system with the column.

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Device Recommended specifications and/or setting
Pump Flow rate: max. 1 mL/min.
Injector Low dead volume type
Sample loop max. 50 μL (5 μL loop is recommended)
From injector to column 0.1 to 0.13 mm I.D., 100 mm Length piping
From column to detector 0.1 mm I.D. piping
Detector

Detector with a micro cell should be used. Response should be set to "FAST".

Sample: (A); 1.5 μL, (B); 5 μL

  1. 1.Methyl ethyl ketone, 2-Butanone 0.2 %
  2. 2.Diethyl ketone, 3-Pentanone 0.2 %
  3. 3.Di-n-propyl ketone, 4-Heptanone 0.4 %
  4. 4.Di-n-butyl ketone, 5-Nonanone 0.6 %
Column
：Shodex RSpak DE-413 (4.6 mm I.D. x 150 mm)
Eluent
：H2O/CH3CN=50/50
Flow rate
：1.0 mL/min
Detector
：UV (262 nm)
Column temp.
：40 ℃

Operation Manual / Certificate of Analysis

Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

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