Since hinokititol and oxin-Cu are strong metal coordination compounds, they are adsorbed with the impurity metals in silica gel and the adsorption causes peak tailing. In case of RSpak DE series (polymer-based columns for reversed phase chromatography), the column is free from the effect of metals and sample will not be adsorbed by the column.


Sample : 5 μL
(Left)
1. 0.01 % Hinokitiol
2. 0.001 % Benzene
(Right)
1. 0.01 % Oxine-Cu

Column       : Shodex RSpak DE-413 (4.6 mm I.D. x 150 mm)
Eluent       : (Left); (1 mM EDTA + 20 mM KH2PO4) aq./CH3CN=60/40
               (Right); 20 mM H3PO4 aq./CH3CN=95/5
Flow rate    : 1.0 mL/min
Detector     : UV (254 nm)
Column temp. : 40 °C