Since hinokititol and oxin-Cu are strong metal coordination compounds, they are adsorbed with the impurity metals in silica gel and the adsorption causes peak tailing. In case of RSpak DE series (polymer-based columns for reversed phase chromatography), the column is free from the effect of metals and sample will not be adsorbed by the column.
Sample: 5 μL
- (Left)
- 1.Hinokitiol 0.01 %
- 2.Benzene 0.001 %
- (Right)
- 1.Oxine-Cu 0.01 %
- Column
- ：Shodex RSpak DE-413 (4.6 mm I.D. x 150 mm)
- Eluent
- ：(Left); (1 mM EDTA + 20 mM KH2PO4) aq./CH3CN=60/40
(Right); 20 mM H3PO4 aq./CH3CN=95/5 - Flow rate
- ：1.0 mL/min
- Detector
- ：UV (254 nm)
- Column temp.
- ：40 ℃
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Product Information (Related Information)
- Features of RSpak DE Series (1) (Comparison with ODS Column)
- Features of RSpak DE Series (3) (Effects of Eluent pH on Analysis Result)
- Features of RSpak DE Series (4) (Reproducibility between Production Lots)
- Features of RSpak DE Series (5) (Selection of HPLC System)
- Features of RSpak DE Series (6) (Effects of Flow Rate on Column Efficiency)
- Features of RSpak DE Series (7) (Effects of Sample Load on Column Efficiency)