For an efficient cleaning, reverse the flow direction.
Method
Set the flow rate at 0.5 mL/min. Introduce 50 mL of acetonitrile or methanol. (Cleaning time: 100 minutes)
For an efficient cleaning, reverse the flow direction.
- (Attention)
- Keeping the Washing solution in the column for a long time will lead to column deterioration. Please replace the Washing solution with the eluent immediately after cleaning.