Please read the column cleaning method for your column.
Asahipak GS-HQ series, Asahipak GS-220 20G, Asahipak GS-320 20G,
Asahipak GF-HQ series, MSpak GF-310 4D, Asahipak GS-310 20G and Asahipak GS-510 20G
For an efficient cleaning, reverse the flow direction.
Flow rate during the cleaningScroll
| Product name | Flow rate | Product name | Flow rate | Product name | Flow rate |
|---|---|---|---|---|---|
| Asahipak GS-220 HQ | ≤ 0.3 mL/min | Asahipak GS-320 HQ | ≤ 0.3 mL/min | Asahipak GS-220 20G | ≤ 3.0 mL/min |
| Asahipak GS-320 20G | ≤ 3.0 mL/min | Asahipak GF-210 HQ | ≤ 0.3 mL/min | Asahipak GF-310 HQ | ≤ 0.3 mL/min |
| Asahipak GF-510 HQ | ≤ 0.3 mL/min | Asahipak GF-7M HQ | ≤ 0.3 mL/min | MSpak GF-310 4D | ≤ 0.15 mL/min |
| Asahipak GS-310 20G | ≤ 3.0 mL/min | Asahipak GS-510 20G | ≤ 3.0 mL/min |
Method 1: Adsorption of hydrophobic compounds (when using aqueous eluent)
Introduce the eluent with a high polar organic solvent (acetonitrile or methanol). Recommended solvent volume to introduce is 5 to 10 times of the column volume.
Method 2: Adsorption of ionic compounds
Introduce the eluent with a higher salt concentration. Recommended solvent volume to introduce is 5 to 10 times of the column volume.
- (Attention)
- Keep the polar organic solvent and salt concentrations within the concentrations stated in the Operation Manual "List of Applicable Solvents".
- Keeping the washing solution in the column for a long time will lead to column deterioration. Please replace the washing solution with the eluent immediately after cleaning.