Cleaning Method: Multimode and Aqueous/Organic SEC Columns

Please read the column cleaning method for your column.

Asahipak GS-HQ series, Asahipak GS-220 20G, Asahipak GS-320 20G,
Asahipak GF-HQ series, MSpak GF-310 4D, Asahipak GS-310 20G and Asahipak GS-510 20G

For an efficient cleaning, reverse the flow direction.

Flow rate during the cleaningScroll
Product name

GS-HQ series

GF-HQ series

GS-220 20G, GS-320 20G

GS-310 20G, GS-510 20G

GF-310 4D
Flow rate ≤ 0.3 mL/min ≤ 3.0 mL/min ≤ 0.15 mL/min

 

Method 1: Adsorption of hydrophobic compounds (when using aqueous eluent)

Introduce the eluent with a high polar organic solvent (acetonitrile or methanol). Recommended solvent volume to introduce is 5 to 10 times of the column volume.

 

Method 2: Adsorption of ionic compounds

Introduce the eluent with a higher salt concentration. Recommended solvent volume to introduce is 5 to 10 times of the column volume.

(Attention)
Keep the polar organic solvent and salt concentrations within the concentrations stated in the Operation Manual "List of Applicable Solvents".
 
Keeping the washing solution in the column for a long time will lead to column deterioration. Please replace the washing solution with the eluent immediately after cleaning.

Operation Manual / Certificate of Analysis

Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

Product Name Index