Cleaning Method: Ion Chromatography Columns (Anion Analysis)

Please read the column cleaning method for your column.
For an efficient cleaning, reverse the flow direction.

IC I-524A and IC NI-424

For an efficient cleaning, reverse the flow direction.

 

Method 1: Adsorption of metal ions

Set the flow rate at 1.0 mL/min. Introduce 30 mL of 0.01-M aqueous tartaric acid solution (pH3 - 4 adjusted by 0.1-M hydroxide sodium). (Cleaning time: 30 minutes)

 

Method 2: Adsorption of proteins

Set the flow rate at 0.2 mL/min. Introduce 30 mL of 20 % (v/v) aqueous acetic acid solution, and then introduce 30 mL of water. (Cleaning time: 150 minutes)

 

Method 3: Adsorption of organic compounds

【NI-424】Set the flow rate at 0.2 mL/min. Introduce 30 mL of acetonitrile/water = 10/90, and then introduce 30 mL of water. (Cleaning time: 30 minutes each)

 

【I-524A】Set the flow rate at 0.2 mL/min. Introduce 30 mL of acetonitrile/water = 20/80, and then introduce 30 mL of water. (Cleaning time: 30 minutes each)

(Attention)
Keeping the Washing solution in the column for a long time will lead to column deterioration. Please replace the Washing solution with the eluent immediately after cleaning.

IC SI-90 4E, IC SI-50 4E, IC SI-52 4E, IC SI-35 4D and IC SI-35 2B

For an efficient cleaning, reverse the flow direction.

Flow rate during the cleaningScroll
Product name SI-90 4E SI-50 4E SI-52 4E SI-35 4D SI-35 2B
Flow rate 0.5 mL/min 0.3 mL/min 0.3 mL/min 0.3 mL/min 0.1 mL/min
Method 1: Adsorption of hydrophilic compounds Scroll
Washing solution Cleaning time (min)
SI-90 4E SI-50 4E SI-52 4E SI-35 4D SI-35 2B
1 H2O 15   25   25 15   4
2 10 x Concentrated Eluent 60 100 100 60 15
3 H2O 15   25   25 15   4
4 Eluent
(Reverse the column flow to normal direction)		 60 100 100 60 15
Method 2: Adsorption of hydrophobic compoundsScroll
Washing solution Cleaning time (min)
SI-90 4E SI-50 4E SI-52 4E SI-35 4D SI-35 2B
1 H2O 15   25   25 15   4
2 H2O/CH3CN = 95/5 10   20   20 12   3
3 CH3CN 60 100 100 60 15
4 H2O 30   50   50 30   8
5 Eluent
(Reverse the column flow to normal direction)		 60 100 100 60 15

 

Method 3: When abnormal nitrate peak (tailing, unsymmetrical etc.) was observed.  (SI-90 4E only)Note: Keep the normal column flow direction.Scroll
Washing solution Cleaning time (min)
1 H2O 15
2 1 M Sodium Carbonate Solution 25
3

Remove the column from the system, tighten the end caps, and store the column at room temperature for 3 days. (Leaving it for more than 4 days can damage the column.)
4 Eluent 60
(Attention)
Keeping the Washing solution in the column for a long time will lead to column deterioration. Please replace the Washing solution with the eluent immediately after cleaning.

IC SI-36 4D and IC SI-37 4D

For an efficient cleaning, reverse the flow direction.

Flow rate during the cleaningScroll
Product name SI-36 4D SI-37 4D
Flow rate 0.35 mL/min 0.25 mL/min
Method 1: Adsorption of hydrophilic compounds Scroll
Washing solution Cleaning time (min)
SI-36 4D SI-37 4D
1 H2O 15 20
2 100 mM KOH or NaOH aq. solution 50 70
3 H2O 15 20
4 Eluent
(Reverse the column flow to normal direction)		 50 70
Method 2: Adsorption of hydrophobic compoundsScroll
Washing solution Cleaning time (min)
SI-36 4D SI-37 4D
1 H2O 15 20
2 H2O/CH3CN = 95/5 10 15
3 CH3CN 50 70
4 H2O 30 40
5 Eluent
(Reverse the column flow to normal direction)		 50 70
(Attention)
Keeping the Washing solution in the column for a long time will lead to column deterioration. Please replace the Washing solution with the eluent immediately after cleaning.
 
Strong alkaline solvents such as 100-mM aqueous sodium hydroxide solution can damage the detector cell. Do not connect the detector while regenerating the column and collect elute directly from the column outlet to the waste container.
Back to Column Cleaning

Operation Manual / Certificate of Analysis

Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

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