Please read the column cleaning method for your column.
For an efficient cleaning, reverse the flow direction.
For an efficient cleaning, reverse the flow direction.
Method 1: Adsorption of metal ions
Set the flow rate at 1.0 mL/min. Introduce 30 mL of 0.01-M aqueous tartaric acid solution (pH3 - 4 adjusted by 0.1-M hydroxide sodium). (Cleaning time: 30 minutes)
Method 2: Adsorption of proteins
Set the flow rate at 0.2 mL/min. Introduce 30 mL of 20 % (v/v) aqueous acetic acid solution, and then introduce 30 mL of water. (Cleaning time: 150 minutes)
Method 3: Adsorption of organic compounds
【NI-424】Set the flow rate at 0.2 mL/min. Introduce 30 mL of acetonitrile/water = 10/90, and then introduce 30 mL of water. (Cleaning time: 30 minutes each)
【I-524A】Set the flow rate at 0.2 mL/min. Introduce 30 mL of acetonitrile/water = 20/80, and then introduce 30 mL of water. (Cleaning time: 30 minutes each)
- (Attention)
- Keeping the Washing solution in the column for a long time will lead to column deterioration. Please replace the Washing solution with the eluent immediately after cleaning.
IC SI-90 4E, IC SI-50 4E, IC SI-52 4E, IC SI-35 4D and IC SI-35 2B
For an efficient cleaning, reverse the flow direction.
| Product name | Flow rate | Product name | Flow rate | Product name | Flow rate |
|---|---|---|---|---|---|
| IC SI-90 4E | 0.5 mL/min | IC SI-50 4E | 0.3 mL/min | IC SI-52 4E | 0.3 mL/min |
| IC SI-35 4D | 0.3 mL/min | IC SI-35 2B | 0.1 mL/min |
| Washing solution | Cleaning time (min) | ||||||
|---|---|---|---|---|---|---|---|
| IC SI-90 4E | IC SI-50 4E | IC SI-52 4E | IC SI-35 4D | IC SI-35 2B | |||
| 1 | H2O | 15 | 25 | 25 | 15 | 4 | |
| 2 | 10 x Concentrated Eluent | 60 | 100 | 100 | 60 | 15 | |
| 3 | H2O | 15 | 25 | 25 | 15 | 4 | |
| 4 | Eluent (Reverse the column flow to normal direction) |
60 | 100 | 100 | 60 | 15 | |
| Washing solution | Cleaning time (min) | ||||||
|---|---|---|---|---|---|---|---|
| IC SI-90 4E | IC SI-50 4E | IC SI-52 4E | IC SI-35 4D | IC SI-35 2B | |||
| 1 | H2O | 15 | 25 | 25 | 15 | 4 | |
| 2 | H2O/CH3CN = 95/5 | 10 | 20 | 20 | 12 | 3 | |
| 3 | CH3CN | 60 | 100 | 100 | 60 | 15 | |
| 4 | H2O | 30 | 50 | 50 | 30 | 8 | |
| 5 | Eluent (Reverse the column flow to normal direction) |
60 | 100 | 100 | 60 | 15 | |
| Washing solution | Cleaning time (min) | ||
|---|---|---|---|
| 1 | H2O | 15 | |
| 2 | 1 M Sodium Carbonate Solution | 25 | |
| 3 |
Remove the column from the system, tighten the end caps, and store the column at room temperature for 3 days. (Leaving it for more than 4 days can damage the column.) |
||
| 4 | Eluent | 60 | |
- (Attention)
- Keeping the Washing solution in the column for a long time will lead to column deterioration. Please replace the Washing solution with the eluent immediately after cleaning.
IC SI-36 4D and IC SI-37 4D
For an efficient cleaning, reverse the flow direction.
| Product name | Flow rate | Product name | Flow rate |
|---|---|---|---|
| IC SI-36 4D | 0.35 mL/min | IC SI-37 4D | 0.25 mL/min |
| Washing solution | Cleaning time (min) | |||
|---|---|---|---|---|
| IC SI-36 4D | IC SI-37 4D | |||
| 1 | H2O | 15 | 20 | |
| 2 | 100 mM KOH or NaOH aq. solution | 50 | 70 | |
| 3 | H2O | 15 | 20 | |
| 4 | Eluent (Reverse the column flow to normal direction) |
50 | 70 | |
| Washing solution | Cleaning time (min) | |||
|---|---|---|---|---|
| IC SI-36 4D | IC SI-37 4D | |||
| 1 | H2O | 15 | 20 | |
| 2 | H2O/CH3CN = 95/5 | 10 | 15 | |
| 3 | CH3CN | 50 | 70 | |
| 4 | H2O | 30 | 40 | |
| 5 | Eluent (Reverse the column flow to normal direction) |
50 | 70 | |
- (Attention)
- Keeping the Washing solution in the column for a long time will lead to column deterioration. Please replace the Washing solution with the eluent immediately after cleaning.
- Strong alkaline solvents such as 100-mM aqueous sodium hydroxide solution can damage the detector cell. Do not connect the detector while regenerating the column and collect elute directly from the column outlet to the waste container.