Presence of cations other than the ion modified on the sulfo functional group may replace the original ion attached. This can change the separation patterns and peak shapes. Regeneration procedure reattaches the original ions and may resume the column performance.
Please read the regeneration procedure specific to your column.
Set the column temperature at 50 ℃ and the flow rate at 0.5 mL/min. First run water, and then introduce 50 mL of 50-mM aqueous sodium hydroxide solution. Wash the column thoroughly with water. (Time: 100 minutes)
Method 2:
Set the column temperature at 50 ℃ and the flow rate at 0.5 mL/min. Introduce water and inject 20 to 50 µL of 0.5-M aqueous sodium hydroxide solution several times.
- (Attention)
- Keeping the regeneration solution in the column for a long time will lead to column deterioration. Please replace the regeneration solution with the eluent immediately after cleaning.
- Strong alkaline solvents such as aqueous sodium hydroxide solution can damage the detector cell. Do not connect the detector while regenerating the column and collect elute directly from the column outlet to the waste container.
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| Solvent (Column temperature: 50 ℃, Flow rate: 0.5 mL/min) | Time (min) | |
|---|---|---|
| 1 | H2O | 30 |
| 2 | 0.1 M Zn(NO3)2 aq. solution | 60 |
| 3 | H2O | 60 |
| 4 | Eluent | 30 |
- (Caution)
- In Japan, zinc ions are subject to the Water Pollution Control Law. Please be careful when disposing the eluate containing zinc ions.
- (Attention)
- Keeping the regeneration solution in the column for a long time will lead to column deterioration. Please replace the regeneration solution with the eluent immediately after cleaning.
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| Solvent (Column temperature: 50 ℃, Flow rate: 0.5 mL/min) | Time (min) | |
|---|---|---|
| 1 | H2O | 30 |
| 2 | 0.1 M Ca(NO3)2 aq. solution | 60 |
| 3 | H2O | 60 |
| 4 | Eluent | 30 |
- (Attention)
- Keeping the regeneration solution in the column for a long time will lead to column deterioration. Please replace the regeneration solution with the eluent immediately after cleaning.