Presence of cations other than the ion modified on the sulfo functional group may replace the original ion attached. This can change the separation patterns and peak shapes. Regeneration procedure reattaches the original ions and may resume the column performance.
Please read the regeneration procedure specific to your column.
SUGAR SC1011, EP SC1011-7F and USPpak MN-431
Set the column temperature at 50 ℃. Introduce 0.1-M aqueous calcium nitrate solution.
| Product name | Flow rate (mL/min) | Regeneration solvent volume to introduce (mL) | Regeneration time (min) |
|---|---|---|---|
| SUGAR SC1011 | 0.5 | 50 | 100 |
| EP SC1011-7F | 0.5 | 50 | 100 |
| USPpak MN-431 | 0.2 | 12 | 60 |
- (Attention)
- Keeping the regeneration solution in the column for a long time will lead to column deterioration. Please replace the regeneration solution with the eluent immediately after cleaning.
Set the column temperature at 70 to 95 ℃ and the flow rate at 0.5 mL/min. Introduce water and inject 50 μL of 0.2-M aqueous lead nitrate solution several times.
- (Caution)
- Please be careful when disposing the eluate containing lead nitrate solution.
Set the column temperature at 50 ℃ and the flow rate at 0.5 mL/min. Introduce 50 mL of 0.2-M aqueous sodium hydroxide solution. Wash the column thoroughly with water. (Time: 100 minutes)
Method 2:
Set the column temperature at 50 ℃ and the flow rate at 0.5 mL/min. Intorudce water and inject 40 µL of 1-M aqueous sodium hydroxide solution.
- (Attention)
- Keeping the regeneration solution in the column for a long time will lead to column deterioration. Please replace the regeneration solution with the eluent immediately after cleaning.
- Strong alkaline solvents such as aqueous sodium hydroxide solution can damage the detector cell. Do not connect the detector while regenerating the column and collect the elute directly from the column outlet to a waste container.