Cleaning Method: Polymer-based Columns for Hydrophilic Interaction Chromatography (HILIC) (HILICpak)

Please read the column cleaning method for your column.

HILICpak VG-25 and VG-50 series

For an efficient cleaning, reverse the flow direction.

Flow rate during the cleaningScroll
Product name VG-25 4D VG-25 2B VG-50 4D VG-50 4E VG-50 2D
Flow rate 0.3 mL/min 0.1 mL/min 0.5 mL/min 0.5 mL/min 0.1 mL/min
Method 1: General cleaningScroll
Washing solution Cleaning time (min)
VG-25 4D VG-25 2B VG-50 4D VG-50 4E VG-50 2D
1 H2O   30   5   6   10   6
2 0.1 M NaOH aq. solution   75 15 75 120 75
3 H2O 100 60 12   20 12
4 Eluent 200 60 40   60 40
Method 2: Adsorption of metal ions or in case Method 1 did not work Scroll
Washing solution Cleaning time (min)
VG-25 4D VG-25 2B VG-50 4D VG-50 4E VG-50 2D
1 H2O   30   5   6   10   6
2 0.1 M HNO3 aq. solution   75 15 75 120 75
3 H2O   30   5  6   10   6
4 0.1 M NaOH aq. solution   75 15 75 120 75
5 H2O 100 60 12   20 12
6 Eluent 200 60 40   60 40
(Attention)
Keeping the washing solution in the column for a long time will lead to column deterioration. Please replace the washing solution with the eluent immediately after cleaning.
 
Strong alkaline solvents such as 0.1-M aqueous sodium hydroxide solution can damage the detector cell. Do not connect the detector while regenerating the column and collect elute directly from the column outlet to the waste container.

HILICpak VT-50 2D

For an efficient cleaning, reverse the flow direction.

Method 1: General cleaning Scroll
Washing solution (Flow rate: 0.1 mL/min) Cleaning time (min)
1 H2O   6
2 0.1 M NaOH aq. solution 75
3 H2O 12
4 Eluent 40
Method 2: Adsorption of metal ions or in case Method 1 did not work Scroll
Washing solution (Flow rate: 0.1 mL/min) Cleaning time (min)
1 H2O   6
2 0.1 M HNO3 aq. solution 75
3 H2O   6
4 0.1 M NaOH aq. solution 75
5 H2O 12
6 Eluent 40
(Attention)
Keeping the washing solution in the column for a long time will lead to column deterioration. Please replace the washing solution with the eluent immediately after cleaning.
 
Strong alkaline solvents such as 0.1-M aqueous sodium hydroxide solution can damage the detector cell. Do not connect the detector while regenerating the column and collect elute directly from the column outlet to the waste container.

HILICpak VC-50 2D

For an efficient cleaning, reverse the flow direction.

MethodScroll
Washing solution (Flow rate:0.1 mL/min) Cleaning time (min)
1 H2O   6
2 0.1 M HNO3 aq. solution 75
3 H2O 12
4 Eluent 40
(Attention)
Keeping the washing solution in the column for a long time will lead to column deterioration. Please replace the washing solution with the eluent immediately after cleaning.

HILICpak VN-50 series

For an efficient cleaning, reverse the flow direction.

Flow rate during the cleaningScroll
Product name VN-50 4D VN-50 2D VN-50 1D VN-50 10E
Flow rate 0.3 mL/min 0.1 mL/min 0.02 mL/min 1.5 mL/min
MethodScroll
Washing solution Cleaning time (min)
VN-50 4D VN-50 2D VN-50 1D VN-50 10E
1 H2O   10   6   6   20
2 0.1 M HNO3 aq. solution 125 75 75 190
3 H2O   10   6   6   20
4 0.1 M NaOH aq. solution 125 75 75 190
5 H2O   20  12 12   30
6 Eluent   70 40 40 100
(Attention)
Keeping the washing solution in the column for a long time will lead to column deterioration. Please replace the washing solution with the eluent immediately after cleaning.
 
Strong alkaline solvents such as 0.1-M aqueous sodium hydroxide solution can damage the detector cell. Do not connect the detector while regenerating the column and collect elute directly from the column outlet to the waste container.
Back to Column Cleaning

Operation Manual / Certificate of Analysis

Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

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