Please read the column cleaning method for your column.
HILICpak VG-50 series
For an efficient cleaning, reverse the flow direction.
Flow rate during the cleaningScroll
| Product name | Flow rate | Product name | Flow rate | Product name | Flow rate |
|---|---|---|---|---|---|
| HILICpak VG-50 4D | 0.5 mL/min | HILICpak VG-50 4E | 0.5 mL/min | HILICpak VG-50 2D | 0.1 mL/min |
Method 1: General cleaningScroll
| Washing solution | Cleaning time (min) | |||
|---|---|---|---|---|
| HILICpak VG-50 4D | HILICpak VG-50 4E | HILICpak VG-50 2D | ||
| 1 | H2O | 6 | 10 | 6 |
| 2 | 0.1 M NaOH aq. solution | 75 | 120 | 75 |
| 3 | H2O | 12 | 20 | 12 |
| 4 | Eluent | 40 | 60 | 40 |
Method 2: Adsorption of metal ions or in case Method 1 did not work Scroll
| Washing solution | Cleaning time (min) | |||
|---|---|---|---|---|
| HILICpak VG-50 4D | HILICpak VG-50 4E | HILICpak VG-50 2D | ||
| 1 | H2O | 6 | 10 | 6 |
| 2 | 0.1 M HNO3 aq. solution | 75 | 120 | 75 |
| 3 | H2O | 6 | 10 | 6 |
| 4 | 0.1 M NaOH aq. solution | 75 | 120 | 75 |
| 5 | H2O | 12 | 20 | 12 |
| 6 | Eluent | 40 | 60 | 40 |
- (Attention)
- Keeping the washing solution in the column for a long time will lead to column deterioration. Please replace the washing solution with the eluent immediately after cleaning.
- Strong alkaline solvents such as 0.1-M aqueous sodium hydroxide solution can damage the detector cell. Do not connect the detector while regenerating the column and collect elute directly from the column outlet to the waste container.
For an efficient cleaning, reverse the flow direction.
Method 1: General cleaning Scroll
| Washing solution (Flow rate: 0.1 mL/min) | Cleaning time (min) | |
|---|---|---|
| 1 | H2O | 6 |
| 2 | 0.1 M NaOH aq. solution | 75 |
| 3 | H2O | 12 |
| 4 | Eluent | 40 |
Method 2: Adsorption of metal ions or in case Method 1 did not work Scroll
| Washing solution (Flow rate: 0.1 mL/min) | Cleaning time (min) | |
|---|---|---|
| 1 | H2O | 6 |
| 2 | 0.1 M HNO3 aq. solution | 75 |
| 3 | H2O | 6 |
| 4 | 0.1 M NaOH aq. solution | 75 |
| 5 | H2O | 12 |
| 6 | Eluent | 40 |
- (Attention)
- Keeping the washing solution in the column for a long time will lead to column deterioration. Please replace the washing solution with the eluent immediately after cleaning.
- Strong alkaline solvents such as 0.1-M aqueous sodium hydroxide solution can damage the detector cell. Do not connect the detector while regenerating the column and collect elute directly from the column outlet to the waste container.
For an efficient cleaning, reverse the flow direction.
MethodScroll
| Washing solution (Flow rate:0.1 mL/min) | Cleaning time (min) | |
|---|---|---|
| 1 | H2O | 6 |
| 2 | 0.1 M HNO3 aq. solution | 75 |
| 3 | H2O | 12 |
| 4 | Eluent | 40 |
- (Attention)
- Keeping the washing solution in the column for a long time will lead to column deterioration. Please replace the washing solution with the eluent immediately after cleaning.
HILICpak VN-50 series
For an efficient cleaning, reverse the flow direction.
Flow rate during the cleaningScroll
| Product name | Flow rate | Product name | Flow rate |
|---|---|---|---|
| HILICpak VN-50 4D | 0.3 mL/min | HILICpak VN-50 1D | 0.02 mL/min |
| HILICpak VN-50 2D | 0.1 mL/min | HILICpak VN-50 10E | 1.5 mL/min |
MethodScroll
| Washing solution | Cleaning time (min) | ||||
|---|---|---|---|---|---|
| HILICpak VN-50 4D | HILICpak VN-50 1D | HILICpak VN-50 2D | HILICpak VN-50 10E | ||
| 1 | H2O | 10 | 6 | 6 | 20 |
| 2 | 0.1 M HNO3 aq. solution | 125 | 75 | 75 | 190 |
| 3 | H2O | 10 | 6 | 6 | 20 |
| 4 | 0.1 M NaOH aq. solution | 125 | 75 | 75 | 190 |
| 5 | H2O | 20 | 12 | 12 | 30 |
| 6 | Eluent | 70 | 40 | 40 | 100 |
- (Attention)
- Keeping the washing solution in the column for a long time will lead to column deterioration. Please replace the washing solution with the eluent immediately after cleaning.
- Strong alkaline solvents such as 0.1-M aqueous sodium hydroxide solution can damage the detector cell. Do not connect the detector while regenerating the column and collect elute directly from the column outlet to the waste container.