Cleaning Method: Polymer-based Columns for Reversed Phase Chromatography (RSpak)

Please read the column cleaning method for your column.

RSpak DS-613, RSpak DS-413, RSpak DE-613, RSpak DE-413 and RSpak DE-213

For an efficient cleaning, reverse the flow direction.

Flow rate during the cleaningScroll
Product name DS-613 DS-413 DE-613 DE-413 DE-213
Flow rate ≤ 0.5 mL/min ≤ 0.3 mL/min ≤ 0.5 mL/min ≤ 0.5 mL/min ≤ 0.1 mL/min

 

Method 1: Basic solvent wash (Apply after using the column under acidic to neutral condition for an extended period of time.)

Introduce 10-mM aqueous sodium hydroxide solution/acetonitrile = 80/20. Recommended solvent volume to introduce is 5 to 10 times of the column volume.

 

Method 2: Acid solvent wash (Apply after using the column under basic to neutral condition for an extended period of time.)

Introduce 50-mM aqueous phosphoric acid solution/acetonitrile = 90/10. Recommended solvent volume to introduce is 5 to 10 times of the column volume.

 

Method 3: Organic solvent wash (Apply after using the column under highly aqueous condition for an extended period of time.）

Introduce water/acetonitrile = 20/80. Recommended solvent volume to introduce is 5 to 10 times of the column volume.

(Attention)

Keeping the washing solution in the column for a long time will lead to column deterioration. Please replace the washing solution with the eluent immediately after cleaning.

RSpak DM-614

Method

For an efficient cleaning, reverse the flow direction.
Set the flow rate at 0.3 mL/min, and introduce 50 mL of methanol, acetonitrile or water. (Cleaning time: 165 minutes)

(Attention)
Keeping the washing solution in the column for a long time will lead to column deterioration. Please replace the washing solution with the eluent immediately after cleaning.

RSpak NN-814

Method: adsorbing ionic componentsIntroduce the eluent and inject 20 - 50 μL of 2-M nitric acid aqueous solution several times.

RSpak JJ-50 2D

For an efficient cleaning, reverse the flow direction.

Method 1: Adsorption of hydrophilic compounds Scroll
Washing solution (Flow rate: 0.1 mL/min) Cleaning time (min)

1

 H2O   6
2 0.1 M NaOH aq. solution 75
3 H2O 12
4 Eluent 40
Method 2: Adsorption of metal ions or in case Method 1 did not workScroll
Washing solution (Flow rate: 0.1 mL/min) Cleaning time (min)
1 H2O   6
2 0.1 M HNO3 aq. solution 75
3 H2O   6
4 0.1 M NaOH aq. solution 75
5 H2O 12
6 Eluent 40
Method 3: Adsorption of hydrophobic compoundsScroll
Washing solution (Flow rate: 0.1 mL/min) Cleaning time (min)
1 H2O   25
2 H2O/CH3CN = 95/5   20
3 CH3CN 100
4 H2O   50
5 Eluent   40
(Attention)
Keeping the washing solution in the column for a long time will lead to column deterioration. Please replace the washing solution with the eluent immediately after cleaning.
 
Strong alkaline solvents such as 0.1-M aqueous sodium hydroxide solution can damage the detector cell. Do not connect the detector while regenerating the column and collect elute directly from the column outlet to the waste container.
Back to Column Cleaning

Operation Manual / Certificate of Analysis

Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

Product Name Index