Cleaning Method: Polymer-based Columns for Reversed Phase Chromatography (RSpak)

Please read the column cleaning method for your column.

RSpak DS-613, RSpak DS-413, RSpak DE-613, RSpak DE-413 and RSpak DE-213

For an efficient cleaning, reverse the flow direction.

Flow rate during the cleaningScroll
Product name Flow rate Product name Flow rate Product name Flow rate
RSpak DS-613 ≤ 0.5 mL/min RSpak DS-413 ≤ 0.3 mL/min RSpak DE-613 ≤ 0.5 mL/min
RSpak DE-413 ≤ 0.5 mL/min RSpak DE-213 ≤ 0.1 mL/min    

 

Method 1: Basic solvent wash (Apply after using the column under acidic to neutral condition for an extended period of time.)

Introduce 10-mM aqueous sodium hydroxide solution/acetonitrile = 80/20. Recommended solvent volume to introduce is 5 to 10 times of the column volume.

 

Method 2: Acid solvent wash (Apply after using the column under basic to neutral condition for an extended period of time.)

Introduce 50-mM aqueous phosphoric acid solution/acetonitrile = 90/10. Recommended solvent volume to introduce is 5 to 10 times of the column volume.

 

Method 3: Organic solvent wash (Apply after using the column under highly aqueous condition for an extended period of time.）

Introduce water/acetonitrile = 20/80. Recommended solvent volume to introduce is 5 to 10 times of the column volume.

(Attention)

Keeping the washing solution in the column for a long time will lead to column deterioration. Please replace the washing solution with the eluent immediately after cleaning.

RSpak DM-614

Method

For an efficient cleaning, reverse the flow direction.
Set the flow rate at 0.3 mL/min, and introduce 50 mL of methanol, acetonitrile or water. (Cleaning time: 165 minutes)

(Attention)
Keeping the washing solution in the column for a long time will lead to column deterioration. Please replace the washing solution with the eluent immediately after cleaning.

RSpak NN-814

Method: adsorbing ionic componentsIntroduce the eluent and inject 20 - 50 μL of 2-M nitric acid aqueous solution several times.

RSpak JJ-50 2D

For an efficient cleaning, reverse the flow direction.

Method 1: Adsorption of hydrophilic compounds Scroll
Washing solution (Flow rate: 0.1 mL/min) Cleaning time (min)

1

 H2O   6
2 0.1 M NaOH aq. solution 75
3 H2O 12
4 Eluent 40
Method 2: Adsorption of metal ions or in case Method 1 did not workScroll
Washing solution (Flow rate: 0.1 mL/min) Cleaning time (min)
1 H2O   6
2 0.1 M HNO3 aq. solution 75
3 H2O   6
4 0.1 M NaOH aq. solution 75
5 H2O 12
6 Eluent 40
Method 3: Adsorption of hydrophobic compoundsScroll
Washing solution (Flow rate: 0.1 mL/min) Cleaning time (min)
1 H2O   25
2 H2O/CH3CN = 95/5   20
3 CH3CN 100
4 H2O   50
5 Eluent   40
(Attention)
Keeping the washing solution in the column for a long time will lead to column deterioration. Please replace the washing solution with the eluent immediately after cleaning.
 
Strong alkaline solvents such as 0.1-M aqueous sodium hydroxide solution can damage the detector cell. Do not connect the detector while regenerating the column and collect elute directly from the column outlet to the waste container.
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