Please read the column cleaning method for your column.
Asahipak ODP-50 series, Asahipak C4P-50 4D and Asahipak ODP-90 20F
For an efficient cleaning, reverse the flow direction.
Flow rate during the cleaningScroll
| Product name | Flow rate | Product name | Flow rate | Product name | Flow rate |
|---|---|---|---|---|---|
| Asahipak ODP-50 6D | ≤ 0.5 mL/min | Asahipak ODP-50 6E | ≤ 0.5 mL/min | Asahipak ODP-50 4B | ≤ 0.4 mL/min |
| Asahipak ODP-50 4D | ≤ 0.5 mL/min | Asahipak ODP-50 4E | ≤ 0.5 mL/min | Asahipak C4P-50 4D | ≤ 0.5 mL/min |
| Asahipak ODP-50 2D | ≤ 0.1 mL/min | Asahipak ODP-50 10E | ≤ 1.0 mL/min | Asahipak ODP-90 20F | ≤ 2.5 mL/min |
Method 1: Basic solvent wash (Apply after using the column under acidic to neutral condition for an extended period of time.)
Introduce 0.1-M aqueous sodium hydroxide solution/acetonitrile = 80/20. Recommended solvent volume to introduce is 5 to 10 times of the column volume.
Method 2: Acid solvent wash (Apply after using the column under basic to neutral condition for an extended period of time.)
Introduce 50-mM aqueous phosphoric acid solution/acetonitrile = 90/10. Recommended solvent volume to introduce is 5 to 10 times of the column volume.
Method 3: Organic solvent wash (Apply after using the column under highly aqueous condition for an extended period of time.)
Introduce water/acetonitrile = 20/80. Recommended solvent volume to introduce is 5 to 10 times of the column volume.
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(Attention)
- Keeping the washing solution in the column for a long time will lead to column deterioration. Please replace the washing solution with the eluent immediately after cleaning.