Please read the column cleaning method for your column.
Asahipak ODP-50 series, Asahipak C4P-50 4D and Asahipak ODP-90 20F
For an efficient cleaning, reverse the flow direction.
Flow rate during the cleaningScroll
| Product name | ODP-50 6D | ODP-50 6E | ODP-50 4B | ODP-50 4D | ODP-50 4E |
|---|---|---|---|---|---|
| Flow rate | ≤ 0.5 mL/min | ≤ 0.5 mL/min | ≤ 0.4 mL/min | ≤ 0.5 mL/min | ≤ 0.5 mL/min |
| Product name | ODP-50 2D | ODP-50 10E | ODP-90 20F | C4P-50 4D |
|---|---|---|---|---|
| Flow rate | ≤ 0.1 mL/min | ≤ 1.0 mL/min | ≤ 2.5 mL/min | ≤ 0.5 mL/min |
Method 1: Basic solvent wash (Apply after using the column under acidic to neutral condition for an extended period of time.)
Introduce 0.1-M aqueous sodium hydroxide solution/acetonitrile = 80/20. Recommended solvent volume to introduce is 5 to 10 times of the column volume.
Method 2: Acid solvent wash (Apply after using the column under basic to neutral condition for an extended period of time.)
Introduce 50-mM aqueous phosphoric acid solution/acetonitrile = 90/10. Recommended solvent volume to introduce is 5 to 10 times of the column volume.
Method 3: Organic solvent wash (Apply after using the column under highly aqueous condition for an extended period of time.)
Introduce water/acetonitrile = 20/80. Recommended solvent volume to introduce is 5 to 10 times of the column volume.
-
(Attention)
- Keeping the washing solution in the column for a long time will lead to column deterioration. Please replace the washing solution with the eluent immediately after cleaning.
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.