Cleaning Method: Polymer-based Columns for Reversed Phase Chromatography (ODP2 HP)

Please read the column cleaning method for your column.

ODP2 HP series

For an efficient cleaning, reverse the flow direction.

Flow rate Scroll
製品名 ODP2 HP-4B ODP2 HP-4D ODP2 HP-4E ODP2 HP-2B ODP2 HP-2D ODP2 HP-10E
流量 0.3 mL/min 0.3 mL/min 0.3 mL/min 0.1 mL/min 0.1 mL/min 0.8 mL/min
Method 1: Basic solvent wash (Apply after using the column under acidic to neutral condition for an extended period of time.)Scroll
Washing solution Cleaning time (min)
ODP2 HP-4B ODP2 HP-4D ODP2 HP-4E ODP2 HP-2B ODP2 HP-2D ODP2 HP-10E
1 H2O   5   10   20   2   6   20
2 10 mM NaOH aq. solution
/CH3CN = 80/20		 40 125 200 25 75 190
3 H2O   5   10   20   2   6   20
4 10 mM CH3COOH aq. solution 40 125 200 25 75 190
5 H2O 10   20   40   5 15   30
6 Eluent 20   70 100 12 40 100
Method 2: Acid solvent wash (Apply after using the column under basic to neutral condition for an extended period of time.) Scroll
Washing solution Cleaning time (min)
ODP2 HP-4B ODP2 HP-4D ODP2 HP-4E ODP2 HP-2B ODP2 HP-2D ODP2 HP-10E
1 H2O   5   10   20   2   6   20
2 50 mM H3PO4 aq. solution
/CH3CN = 90/10		 40 125 200 25 75 190
3 H2O 10   20   40   5 15   30
4 Eluent 20   70 100 12 40 100
Method 3: Organic solvent wash (Apply after using the column under highly aqueous condition for an extended period of time.) Scroll
Washing solution Cleaning time (min)
ODP2 HP-4B ODP2 HP-4D ODP2 HP-4E ODP2 HP-2B ODP2 HP-2D ODP2 HP-10E
1 H2O   5   10   20   2   6   20
2 H2O/CH3CN=20/80 40 125 200 25 75 190
3 H2O 10   20   40   5 15   30
4 Eluent 20   70 100 12 40 100
(Attention)
Keeping the washing solution in the column for a long time will lead to column deterioration. Please replace the washing solution with the eluent immediately after cleaning.
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Operation Manual / Certificate of Analysis

Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

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