Melamine (1,3,5-Triazine-2,4,6-triamine), a compound fraudulently used to falsely increase protein content in milk, is a toxic substance known to cause bladder and kidney stones in humans when chronically ingested. The discovery of melamine contamination in baby formula and in other food products has prompted the need for the development of suitable analytical methods for this substance. This chromatogram shows an application based on HILIC mode using Asahipak NH2P-50 4E .


Sample : Melamine 0.5 mg/L, 5 μL

Column       : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
Eluent       : CH3CN/H2O=75/25
Flow rate    : 1.0 mL/min
Detector     : UV (230 nm)
Column temp. : 30 °C