Vitamin A palmitate was analyzed using GPC KF-801. Wave length of 325 nm at which it has maximum UV absorption was used for the detection. Usually, saponification is necessary to analyze vitamin A as the sample pretreatment. However, in this analysis, no complicated sample pretreatment procedure was necessary. What is necessary is just to dissolve the sample in the eluent (THF) and filtrate it using a 0.45 μm disposable filter. This method using KF-801 is a very simple way to analyze vitamin A.
Sample : Margarine 0.2 %, 100 μL
1. Vitamin A palmitate
Columns : Shodex GPC KF-801 (8.0 mm I.D. x 300 mm) x 2 Eluent : THF Flow rate : 1.0 mL/min Detector : UV (325 nm) Column temp. : 40 °C
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Operation Manual / Certificate of Analysis
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Applications
- Water-Soluble Vitamins (1) (DE-413)
- Water-Soluble Vitamins (4) (DM-614)
- Water-Soluble Vitamins (5) (NH2P-50 4E)
- Simultaneous Analysis of Water-Soluble Vitamins (DE-413)
- LC/MS Analysis of Water-soluble Vitamins (DE-213)
- Vitamin B6 (1) (ODP-50 6D)
- Vitamin B12 (SB-802.5 HQ)
- Vitamin C (1) (KC-811)
- Vitamin C (3) (NH2P-50 4E)
- Soft Drink Containing Vitamin C (NH2P-50 4E)
- LC/MS Analysis of Pantothenic Acid and Vitamin C (VT-50 2D)
- Separation of Ascorbic Acid and Isoascorbic Acid (DE-413)
- Analysis of Ascorbic Acid Injection According to USP-NF Method (DM-614)
- Carnitine (NN-814)
- LC/MS Analysis of Carnitine and Trimethyl Glycine (NH2P-40 2D)
- Food Containing Vitamins (DE-413)
- Nutrient Beverage (DE-413)
- Separation of Water-Soluble Vitamin and Sulfite (KC-811)
- Vitamin D (ODP-50 6E)
- Fat-Soluble Vitamins (1) (ODP-50 4E)
- Fat-Soluble Vitamins (3) (KF-801)
- Carotene Analysis in Carrot Juice (C18U 2D)