Vitamin D2 and D3 were separated using Asahipak ODP-50 6E, a polymer-based reversed phase column. ODP-50 has a wide pH range and can be washed with an aqueous solution of acid or alkali, so it is a highly durable column even for samples with a lot of impurities.
Sample : 4 IU (0.1 μg)/mL each, 100 μL
1. Vitamin D2 (Ergocalciferol)
2. Vitamin D3 (Cholecaciferol)
Column : Shodex Asahipak ODP-50 6E (6.0 mm I.D. x 250 mm) Eluent : CH3CN/CH3OH=90/10 Flow rate : 1.5 mL/min Detector : UV (265 nm) Column temp. : 27 °C
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Water-Soluble Vitamins (1) (DE-413)
- Water-Soluble Vitamins (4) (DM-614)
- Water-Soluble Vitamins (5) (NH2P-50 4E)
- Simultaneous Analysis of Water-Soluble Vitamins (DE-413)
- LC/MS Analysis of Water-soluble Vitamins (DE-213)
- Vitamin B6 (1) (ODP-50 6D)
- Vitamin B12 (SB-802.5 HQ)
- Vitamin C (1) (KC-811)
- Vitamin C (3) (NH2P-50 4E)
- Soft Drink Containing Vitamin C (NH2P-50 4E)
- LC/MS Analysis of Pantothenic Acid and Vitamin C (VT-50 2D)
- Separation of Ascorbic Acid and Isoascorbic Acid (DE-413)
- Analysis of Ascorbic Acid Injection According to USP-NF Method (DM-614)
- Carnitine (NN-814)
- LC/MS Analysis of Carnitine and Trimethyl Glycine (NH2P-40 2D)
- Food Containing Vitamins (DE-413)
- Nutrient Beverage (DE-413)
- Separation of Water-Soluble Vitamin and Sulfite (KC-811)
- Margarine (KF-801)
- Fat-Soluble Vitamins (1) (ODP-50 4E)
- Fat-Soluble Vitamins (3) (KF-801)
- Carotene Analysis in Carrot Juice (C18U 2D)