LC/MS Analysis of Pantothenic Acid and Vitamin C (VT-50 2D)

Pantothenic acid and ascorbic acid were analyzed by HILICpak VT-50 2D under the conditions of HILIC mode LC/MS.


Sample : 100 ng/mL, 10 μL
1. Pantothenic acid (Vitamin B5)
2. Ascorbic acid (Vitamin C)


Column       : Shodex HILICpak VT-50 2D (2.0 mm I.D. x 150 mm)
Eluent       : 50 mM HCOONH4 aq./CH3CN=30/70
Flow rate    : 0.2 mL/min
Detector     : ESI-MS (SIM)
Column temp. : 30 °C

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