Polyamines were analyzed by on-column derivertization using Asahipak ODP-50 4D.
Refer to Instruments for On-Column Derivetization


Sample : 10 μL
Histamine (Him) , Diethylenetriamine (Det:I.S.) , Tyramine (Tym) , 1,3-Diaminopropane (Dap)
Putrescine (Put) , Spermidine (Spd) , Spermine (Spm) , Cadaverine (Cad)

Column       : Shodex Asahipak ODP-50 4D (4.6 mm I.D. x 150 mm)
Eluent       : 50 mM Borate buffer (pH9.9) - CH3CN(77:23,v/v)
               containing 2 mM OPA(o-Phthalaldehyde) and 2 mM NAC(N-Acetyl-L-cysteine)
Flow rate    : 0.5 mL/min
Detector     : Fluorescence (Ex. 330 nm, Em. 430 nm)
Column temp. : 40 °C

K.Saito, M.Horie, N.Nose,K.Nakagomi and H.Nakazawa, Anal. Sci. 8,675(1992)