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LC/MS Analysis of Choline and Acetylcholine (VC-50 2D)

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Choline and acetylcholine were analyzed by LC/MS method using HILICpak VC-50 2D, a polymer-based HILIC column. The calibration curves of choline and acetylcholine show good linearity respectively in the range from 1 to 100mM.


Sample : 5μL
5nM each (in H2O)
1. Acetylcholine
2. Choline



Column       : Shodex HILICpak VC-50 2D (2.0mm I.D. x 150mm)
Eluent       : 200mM HCOOH aq./CH3CN=40/60
Flow rate    : 0.3mL/min
Detector     : ESI-MS/MS (MRM Positive)
Column temp. : 40°C

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