Thermally unstable and/or involatile agricultural chemicals which cannot be analyzed by GC or GC/MS can be analyzed by LC/MS.
A semi-micro column RSpak DE-213 was used for the separation.


Sample : 20 ng each, 20 μL
1. Asulam
2. 2-(4-Chloro-2-methylphenoxy)propanoic acid, MCPP
3. Thiuram
4. Bensulide, SAP
5. Iprodione
6. Pencycuron

(HPLC)
Instrument   : Agilent 1100
Column       : Shodex RSpak DE-213 (2.0 mm I.D. x 150 mm)
Eluent       : (A): 50 mM CH3COONH4 aq.
               (B): CH3CN
               0 min to 10 min, (B)10 % to (B)100 %
Flow rate    : 0.2 mL/min
Column temp. : 40 °C
(MS)
Instrument   : Agilent 1100MSD
Mass range   : 105-500 (m/z)
Ionization   : Electrospray
Polarity     : Positive, Negative
Fragmentor   : 60 V
Nebulizer    : N2 (50psi)
Drying gas   : N2 (12 L/min, 350 °C)