For an efficient cleaning, reverse the flow direction.
Method 1: Adsorption of hydrophobic compounds (when using aqueous eluent)
For an efficient cleaning, reverse the flow direction. Introduce the eluent with higher acetonitrile or methanol concentration. Set the flow rate at less than 0.2 mL/min. Recommended solvent volume to introduce is 5 to 10 times of the column volume.
Method 1: Adsorption of hydrophobic compounds (when using aqueous eluent)
For an efficient cleaning, reverse the flow direction. Introduce the eluent with higher salt concentration. Set the flow rate at less than 0.2 mL/min. Recommended solvent volume to introduce is 5 to 10 times of the column volume.
- (Attention)
- Keep the polar organic solvent and salt concentrations within the concentrations stated in the Operation Manual "List of Applicable Solvents".
- Keeping the washing solution in the column for a long time will lead to column deterioration. Please replace the washing solution with the eluent immediately after cleaning.