Please read the column cleaning method for your column.
For an efficient cleaning, reverse the flow direction.
Method 1: Adsorption of hydrophobic compounds Scroll
| Washing solution (Flow rate: 0.5 mL/min) | Cleaning time (min) | |
|---|---|---|
| 1 | H2O | 10 |
| 2 | 4 mM Methanesulfonic acid aq. solution/CH3CN = 70/30 | 40 |
| 3 | H2O | 10 |
| 4 | Eluent | 40 |
Method 2: Adsorption of hydrophilic compoundsScroll
| Washing solution (Flow rate: 0.5 mL/min) | Cleaning time (min) | |
|---|---|---|
| 1 | H2O | 10 |
| 2 | 20 mM Methanesulfonic acid aq. solution | 40 |
| 3 | H2O | 20 |
| 4 | Eluent | 40 |
- (Attention)
- Keeping the Washing solution in the column for a long time will lead to column deterioration. Please replace the Washing solution with the eluent immediately after cleaning.
For an efficient cleaning, reverse the flow direction.
Method 1: Adsorption of hydrophobic compounds
Set the flow rate at 0.3 mL/min. Introduce 50 mL of a washing solution prepared of 5-mM aqueous tartaric acid solution/acetonitrile = 50/50. (Cleaning time: 165 minutes)
Method 2: Adsorption of hydrophilic compounds
Set the flow rate at 0.3 mL/min. Introduce 50 mL of 50-mM aqueous tartaric acid solution. (Cleaning time: 165 minutes)
- (Attention)
- Keeping the Washing solution in the column for a long time will lead to column deterioration. Please replace the Washing solution with the eluent immediately after cleaning.