Please read the column cleaning method for your column.
SUGAR SH1011, SUGAR SH1821 and SUGAR SH1011 8C
Presence of cations other than the ion modified on the sulfo functional group may replace the original ion attached. This can change the separation patterns and peak shapes. Regeneration procedure reattaches the original ions and may resume the column performance.
Please read the regeneration procedure specific to your column.
Regeneration Method
Set the column temperature at 50 ℃. Introduce 25-mM aqueous sulfurinc acid solution.
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| Product name | Flow rate (mL/min) | Regeneration solvent volume to introduce (mL) | Regeneration time (min) |
|---|---|---|---|
| SUGAR SH1011 | 0.5 | 50 | 100 |
| SUGAR SH1821 | 0.5 | 50 | 100 |
| SUGAR SH1011 8C | 0.5 | 20 | 40 |
- (Attention)
- Keeping the regeneration solution in the column for a long time will lead to column deterioration. Please replace the regeneration solution with the eluent immediately after cleaning.
RSpak KC811 and RSpak KC-811 6E
For an efficient cleaning, reverse the flow direction.
Cleaning method 1: Adsorption of ionic compounds (cations)
Set the column temperature at 50 ℃. Introduce 25-mM aqueous sulfuric acid solution.
Cleaning method 2: Adsorption of hydrophobic compounds
Set the column temperature at 50 ℃. Introduce eluent with 20 % (v/v) acetonitrile or ethanol.
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| Product name | Flow rate (mL/min) | Cleaning solvent volume to introduce (mL) | Cleaning time (min) |
|---|---|---|---|
| RSpak KC-811 | 0.5 | 50 | 100 |
| RSpak KC-811 6E | 0.5 | 25 | 50 |
- (Caution)
- After using a strong acidic eluent such as sulfuric acid solution, wash the HPLC system and the column thoroughly. If left, the metal parts of the system and/or the column may react with the acid and generate hydrogen gas.
- (Attention)
- Keeping the solution in the column for a long time will lead to column deterioration. Please replace the solution with the eluent immediately after cleaning.