Cleaning Method: Silica-based Reversed Phase Chromatography Columns (ODS Columns for UHPLC)

Please read the column cleaning method for your column.

C18U series

For an efficient cleaning, reverse the flow direction.

Flow rate during the cleaningScroll
Product name C18U 2B C18U 2D
Flow rate 0.2 mL/min 以下 0.2 mL/min 以下

 

Method 1: Apply after using the column under an eluent without a buffer or salt.

Introduce the eluent with increased organic solvent content. Up to 100 % organic solvent can be used. Addition of THF may be effective for removing the adsorped lipid conponents. Recommended solvent volume to introduce is 5 to 10 times of the column volume.

 

Method 2: Apply after using the column under an eluent with a buffer or salt.

Replace the eluent with an eluent without the buffer or salt (keeping the same aqueous/organic solvent ratio). Then follow the Method 1. Recommended solvent volume to introduce is 5 to 10 times of the column volume.

(Attention)
Washing the column with 100 % water after using the column with an eluent close to the pH limit may damage the column. Wash the column with a mixture of water and organic solvent in this case.

Operation Manual / Certificate of Analysis

Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

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