Cleaning Method: Polymer-based Columns for Hydrophilic Interaction Chromatography (HILIC) (Asahipak)

Please read the column cleaning method for your column.

Asahipak NH2P-50 series, Asahipak NH2P-40 series and Asahipak NH2P-90 20F

For an efficient cleaning, reverse the flow direction.

Flow rate during the cleaningScroll
Product name Flow rate Product name Flow rate
Asahipak NH2P-50 4B 0.5 mL/min Asahipak NH2P-50 4D 0.5 mL/min
Asahipak NH2P-50 4E 0.5 mL/min Asahipak NH2P-40 3E 0.17 mL/min
Asahipak NH2P-50 2D 0.1 mL/min Asahipak NH2P-40 2E 0.1 mL/min
Asahipak NH2P-50 10E 1.5 mL/min Asahipak NH2P-90 20F 5.0 mL/min

 

Method 1: General cleaning Scroll
Washing solution Cleaning time (min)
Asahipak NH2P-50 4B Asahipak NH2P-50 4D Asahipak NH2P-50 4E Asahipak NH2P-40 3E
1 H2O   2   6   10   13
2 0.1 M NaOH aq. solution 24 75 120 150
3 H2O   4 12   20   26
4 Eluent 12 40   60   75
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Washing solution Cleaning time (min)
Asahipak NH2P-50 2D Asahipak NH2P-40 2E Asahipak NH2P-50 10E Asahipak NH2P-90 20F
1 H2O   6   10   20   25
2 0.1 M NaOH aq. solution 75 120 190 270
3 H2O 12   20   30   50
4 Eluent 40   60 100 140
Method 2: Adsorption of metal ions or in case Method 1 did not work Scroll
Washing solution Cleaning time (min)
Asahipak NH2P-50 4B Asahipak NH2P-50 4D Asahipak NH2P-50 4E Asahipak NH2P-40 3E
1 H2O   2   6   10   13
2 0.1 M HNO3 aq. solution 24 75 120 150
3 H2O 2 6   10   13
4 0.1 M NaOH aq. solution 24 75 120 150
5 H2O   4 12   20   26
6 Eluent 12 40   60   75
Scroll
Washing solution Cleaning time (min)
Asahipak NH2P-50 2D Asahipak NH2P-40 2E Asahipak NH2P-50 10E Asahipak NH2P-90 20F
1 H2O   6   10   20   25
2 0.1 M HNO3 aq. solution 75 120 190 270
3 H2O   6   10   20   25
4 0.1 M NaOH aq. solution 75 120 190 270
5 H2O 12   20   30   50
6 Eluent 40   60 100 140
(Attention)
Keeping the washing solution in the column for a long time will lead to column deterioration. Please replace the washing solution with the eluent immediately after cleaning. 
 
Strong alkaline solvents such as 0.1-M aqueous sodium hydroxide solution can damage the detector cell. Do not connect the detector while regenerating the column and collect elute directly from the column outlet to the waste container.
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