Cleaning Method: Polymer-based Columns for Hydrophilic Interaction Chromatography (HILIC) (Asahipak)

Please read the column cleaning method for your column.

Asahipak NH2Pseries

For an efficient cleaning, reverse the flow direction.

Flow rate during the cleaningScroll
Product name NH2P-50 4B NH2P-50 4D NH2P-50 4E NH2P-40 3E
Flow rate 0.5 mL/min 0.5 mL/min 0.5 mL/min 0.17 mL/min
Product name NH2P-50 2D NH2P-40 2E NH2P-50 10E NH2P-90 20F
Flow rate 0.1 mL/min 0.1 mL/min 1.5 mL/min 5.0 mL/min

 

Method 1: General cleaning Scroll
Washing solution Cleaning time (min)
NH2P-50 4B NH2P-50 4D NH2P-50 4E NH2P-40 3E
1 H2O   2   6   10   13
2 0.1 M NaOH aq. solution 24 75 120 150
3 H2O   4 12   20   26
4 Eluent 12 40   60   75
Scroll
Washing solution Cleaning time (min)
NH2P-50 2D NH2P-40 2E NH2P-50 10E NH2P-90 20F
1 H2O   6   10   20   25
2 0.1 M NaOH aq. solution 75 120 190 270
3 H2O 12   20   30   50
4 Eluent 40   60 100 140
Method 2: Adsorption of metal ions or in case Method 1 did not work Scroll
Washing solution Cleaning time (min)
NH2P-50 4B NH2P-50 4D NH2P-50 4E NH2P-40 3E
1 H2O   2   6   10   13
2 0.1 M HNO3 aq. solution 24 75 120 150
3 H2O 2 6   10   13
4 0.1 M NaOH aq. solution 24 75 120 150
5 H2O   4 12   20   26
6 Eluent 12 40   60   75
Scroll
Washing solution Cleaning time (min)
NH2P-50 2D NH2P-40 2E NH2P-50 10E NH2P-90 20F
1 H2O   6   10   20   25
2 0.1 M HNO3 aq. solution 75 120 190 270
3 H2O   6   10   20   25
4 0.1 M NaOH aq. solution 75 120 190 270
5 H2O 12   20   30   50
6 Eluent 40   60 100 140
(Attention)
Keeping the washing solution in the column for a long time will lead to column deterioration. Please replace the washing solution with the eluent immediately after cleaning. 
 
Strong alkaline solvents such as 0.1-M aqueous sodium hydroxide solution can damage the detector cell. Do not connect the detector while regenerating the column and collect elute directly from the column outlet to the waste container.
Back to Column Cleaning

Operation Manual / Certificate of Analysis

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