使用手性分离色谱柱ORpak CDBS-453对肾上腺素进行分析，结果表明 (R)-肾上腺素和 (S)-肾上腺素的分离度大于 1.5。
Sample: 10 μL
Epinephrine hydrochloride 20 μg/mL (in *Solution A)
- 1.(R)-Epinephrine
- 2.(S)-Epinephrine
- Column
- ：Shodex ORpak CDBS-453 (4.6 mm I.D. x 150 mm)
- Eluent
- ：*Solution A/CH3CN=99/1
*0.75 g/L Ammonium acetate aqueous solution adjusted to pH 4.0 with Glacial acetic acid - Flow rate
- ：0.3 mL/min
- Detector
- ：UV (280 nm)
- Column temp.
- ：25 ℃