Polymer-based-amino column, Asahipak NH2P-50 4E was used to separate stevioside and rebaudioside A, that were extracted from stevia.
Sample : 0.05 % each, 20 μL
1. Stevioside
2. Rebaudioside A
Column : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm) Eluent : H2O/CH3CN=25/75 Flow rate : 1.0 mL/min Detector : UV (210 nm) Column temp. : 30 °C
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Acesulfame K (SC1011)
- Analysis of Acesulfame K and Sucralose (SC1011)
- Sucralose (SP0810)
- LC/MS Analysis of Various Steviol Glycosides (NH2P-40 2D)
- Quantification of Purified Stevia Extract (VG-50 4E)
- Steviol Purity Test of Purified Stevia Extracts According to Japanese Pharmaceutical Excipients Method (C18M 4E)
- Analysis of Mogroside V in Momordica Grosvenori (NH2P-50 4E)
- LC/MS Analysis of Various Sweeteners (NH2P-40 2D)
- Simultaneous Analysis of Sweeteners and Amino Acids in Energy Drink (NH2P-40 2D)
- Simultaneous Analysis of Saccharides and Sweeteners in Yogurt Drink (NH2P-40 2D)
- Effects of Eluent pH on HILIC Mode (VG-50 2D)
- Analysis of Thaumatin (SP-825)