Ribonuclease A from equine pancreas was analyzed using IEC CM-825 (a column for weak cation exchange chromatography). This enzyme contains rather small impurities.


Sample: 0.5 % Ribonuclease A (pI=9.3), 30 μL

Column       : Shodex IEC CM-825 (8.0 mm I.D. x 75 mm)
Eluent       : (A); 20 mM Sodium phosphate buffer (pH7.0)
               (B); (A) + 0.5 M NaCl
               Linear gradient: 0 min to 60 min, (A) to (B)
Flow rate    : 1.0 mL/min
Detector     : UV (280 nm)
Column temp. : 25 °C