Purified Kallikrein from Pig Pancreas was analyzed using IEC QA-825 ( a column for strong anion exchange chromatography ). Enzyme activity was found at the large peak which has two peak tops after 30 minutes. The recovery of enzyme activity was 95%.


Sample : 0.5 % Kallikrein, 200 μL

Column       : Shodex IEC QA-825 (8.0 mm I.D. x 75 mm)
Eluent       : (A); 20 mM Tris-HCl buffer (pH7.5)
               (B); (A) + 0.5 M NaCl
               Linear gradient: 0 min to 60 min, (A) to (B)
Flow rate    : 1.0 mL/min
Detector     : UV (280 nm)
Column temp. : 25 °C