使用聚合物基质反相色谱柱Asahipak ODP-50 4E分析多胺和乙酰衍生物。


Sample :
1. Putrescine
2. Acetylputrescine
3. Cadaverine
4. Spermidine
5. Acetylcadaverine
6. N¹-Acetylspermidine
7. N⁸-Acetylspermidine
8. Spermine
9. Acetylspermine

Column       : Shodex Asahipak ODP-50 4E (4.6 mm I.D. x 250 mm)
Eluent       : 200 mM Sodium phosphate (pH7.7) + 2.3 mM Sodium 1-octanesulfonate 
Flow rate    : 0.5 mL/min
Detector     : Fluoresence (Ex. 345 nm, Em. 455 nm) (OPA post-column reaction)
Column temp. : Room temp.

Courtesy of Dr. Kamei, The Tokyo Metropolitan Institute od Medical Science