使用聚合物基质氨基柱HILICpak VN-50 4D分析半乳糖醛酸的低聚物。由于半乳糖醛酸具有羧基,因此需要使用酸性流动相以抑制解离。使用1%甲酸水溶液和乙腈的梯度条件,用蒸发光散射检测器(ELSD)检测,可以分析至11量体且峰型尖锐。
Sample: 10 μL
Oligogalacturonic acids 5 mg/mL (in 50 % CH3CN)
- 1.Galacturonic acid 7.Oligogalacturonic acid heptamer
- 2.Oligogalacturonic acid dimer 8.Oligogalacturonic acid octamer
- 3.Oligogalacturonic acid trimer 9.Oligogalacturonic acid nonamer
- 4.Oligogalacturonic acid tetramer 10.Oligogalacturonic acid decamer
- 5.Oligogalacturonic acid pentamer 11.Oligogalacturonic acid undecamer
- 6.Oligogalacturonic acid hexamer
- Column
- :Shodex HILICpak VN-50 4D (4.6 mm I.D. x 150 mm)
- Eluent
- :(A); 1 % HCOOH aq., (B); CH3CN
Linear gradient; (B %) 55 to 35 % (0 to 10 min), 35% (10 to 15 min) - Flow rate
- :0.6 mL/min
- Detector
- :ELSD
- Column temp.
- :40 ℃