CXpak P-421S是氨基酸分析专用柱。以下是使用茚三酮显色法分析氨基酸的谱图。17种成分可以在50分钟内分析完毕。


Sample : 0.1 µM each,
100 µL
1. Asp
2. Thr
3. Ser
4. Glu
5. Pro
6. Gly
7. Ala
8. Cystine
9. Val
10. Met
11. Ile
12. Leu
13. Tyr
14. Phe
15. Lys
16. NH₃
17. His
18. Arg

Column       : Shodex CXpak P-421S (4.6 mm I.D. x 150 mm)
Eluent       : MCI BUFFER™ PH Kit (Mitsubishi Chemical Corporation)
               Low pressure step gradient; 
               PH‐1 (0 min), PH‐2 (0.2 min), PH‐3 (13.5 min), PH‐4 (23.2 min), 
               PH‐RG (47.0 min)
Reagent      : Ninhydrin Coloring Solution Kit for HITACHI
               (FUJIFILM Wako Pure Chemical Corporation)
               R1:R2=50:50
Flow rate    : (Eluent) 0.5 mL/min
               (Reagent) 0.35 mL/min
Detector     : VIS (570 nm)
Column temp. : 63 ℃
Reaction temp. : 120 ℃