Humic and fulvic acid extracts prepared from brown forest soil (Danto, Japan) and crumbly black topsoil (Inogashira, Japan) standard samples (provided by the Japanese humic susbtances society) were analyzed by size exclusion chromatography. Under these conditions, humic acids were found have a wide size distribution, while fulvic acid is much less dispersed than humic acids, as show by the molecular weight profiles. OHpak SB-805 HQ is a polyhydroxymethacrylate based size exclusion column with an exclusion limit of 4,000,000 MW (determined with a pullulan standard). It supports a wide pH range (pH 3-10), is compatible with organic solvents such as acetonitrile and methanol, and is used for the analysis of water-soluble macromolecules.
Sample : Humic substances , 30 μL
Humic acid 0.02 mg/mL
Fulvic acid 0.02 mg/mL
Columns : Shodex OHpak SB-G 6B (6.0 mm I.D. x 50 mm) + SB-805 HQ (8.0 mm I.D. x 300 mm) Eluent : 10 mM NaH2PO4 + 10 mM Na2HPO4 (pH7.0) + 25 % CH3CN Flow rate : 0.8 mL/min Detector : UV (260 nm) Column temp. : 40 °C
Data courtesy of Associate Professor Nobuhide Fujitake,
Student Affairs Section, Graduate School of Agricultural Science, Kobe University
Sample Name Index
Operation Manual / Certificate of Analysis
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Product Name Index
Applications
- Mild Hydrolysates of Allosamidin (ES-502C 7C)
- 5-(Hydroxymethyl)Furfural in Fruit Juice (ODP-50 6D)
- Cyanate and Carbamyl Phosphate (ES-502N 7C)
- Hydrogen Peroxide (KS-801)
- Chelating Reagents (1) (I-524A)
- Chelating Reagents (2) (NI-424)
- Analysis of Chelating Agents (ODP-50 4D)
- Hydantoins (DE-613)
- Amides (2) (Comparison of Reversed Phase Columns)
- Amides (3) (DE-413)
- Amides (4) (DE-413)
- Amides (5) (DE-413)
- Aldehydes (DE-413)
- DNPH Derivative Aldehydes (ODP-50 4D)
- Analysis of Pochonicine and Its Analogues in Filamentous Fungi Culture Extract (ES-502C 7C)
- Separation of Furfural and Guaiacol (DE-413)
- Separation of C1 Compounds (DE-413)
- Simultaneous Analysis of Various Nitrogen Compounds According to Japanese Testing Methods for Fertilizers (ES-502C 7C)
- Separation of Substances Produced During Utilization of Biomass (VN-50 4D)
- LC/MS Analysis of Hexamethylenetetramine (VC-50 2D)