Ethylenediamine (0.1 % v/v of 50 mg/mL) is added to water samples to prevent HCl production from the reaction between residual chloride and chlorous acid. Ion chromatography (cation) column, IC YS-50 was used to separate mono- and di-valent cations in the presence of ethylenediamine. 10 % ACN was added to the mobile phase to shorten ethylenediamine retention. Addition of crown ether can improve the separation of Na+ and NH4+ , but this will increase the ethylenediamine retention.
Sample : Cation std.,10 μL
1. Li+ 2 mg/L
2. Na+ 10 mg/L
3. NH4+ 10 mg/L
4. K+ 20 mg/L
5. Mg2+ 10 mg/L
6. Ca2+ 20 mg/L
7. Ethylenediamine, EDA 50 mg/L
Column : Shodex IC YS-50 (4.6 mm I.D. x 125 mm) Eluent : (A); 4 mM Nitric acid aq./CH3CN=90/10 (B); 4 mM Nitric acid + 1.5 mM 18-Crown 6-ether aq./CH3CN=90/10 Flow rate : 1.0 mL/min Detector : Non-suppressed conductivity Column temp. : 40 °C
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Monovalent and Divalent Cation Standards (1) (YK-421)
- Monovalent and Divalent Cation Standards (2) (YK-421)
- Monovalent and Divalent Cation Standards (3) (Dipicolinic Acid + Crown Ether Eluent) (YK-421)
- Monovalent and Divalent Cation Standards (4) (YK-421) (Phosphoric Acid Eluent)
- Monovalent and Divalent Cation Standards (5) (YK-421) (Phosphoric Acid + 18-Crown 6-Ether Eluent)
- Monovalent and Divalent Cation Standards (6) (Comparison of YS-50 and YK-421)
- Monovalent and Divalent Cation Standards (7) (Eluent Conteinig Acetonitrile) (YS-50)
- Dynamic Range of Column (YK-421)
- High Sensitive Detection of Cations (YK-421)
- Effects of Acetonitrile Concentration on Elution Volume (YK-421)
- Effects of Methanesulfonic Acid Concentration on Elution Time (YS-50)
- Effects of Flow Rate on Plate Number (YS-50)
- Effects of Temperature (YS-50)
- Effects of Sample Load on Plate Number (YS-50)
- Linearity of Calibration Curves (YS-50)
- Effects of Crown Ethers (YS-50)
- Tap Water (1) (YK-421)
- Analysis of Tap Water (2) (YK-412) (Phosphoric Acid Eluent)
- Monovalent and Divalent Cations Analysis in Tap Water (Ethylenediamine Containing Sample) (YS-50)
- Monovalent and Divalent Cations Analysis in River Water (Ethylenediamine Containing Sample) (YS-50)
- Mineral Water (YS-50)
- Sea Water (2) (YS-50)
- Red Wine (1) (YK-421)
- Red Wine (3) (YS-50)
- Serum (YK-421)
- Long Chain Alkylamines (YK-421)
- Trialkylamines (YK-421)
- Tetraalkylammoniums (YK-421)
- Analysis of Tetraalkylammoniums (YS-50)
- Alkylamines (2) (YK-421) (Phosphoric Acid + Acetonitrile)
- Ethanolamines and Alkylamines (YK-421) (Phosphoric Acid + Acetonitrile)
- Analysis of Ethanolamines and Alkylamines (YS-50)
- Cations and Alkylamines (YS-50)
- Analysis of Methylamines (YS-50)
- Analysis of Ethylamines (YS-50)
- Analysis of Short Chain Alkylamines (YS-50)
- Cation Analysis in the Presence of PEG (YS-50)
- Cation Analysis in the Presence of Albumin (YS-50)
- Quantification of N-Methylpyrrolidine in Cefepime for Injection According to USP-NF Method (YK-421)