弱陰イオン交換クロマトグラフィー用カラムIEC DEAE-825を用いてヌクレオチドの分離を行いました。
Sample :
1. CMP
2. AMP
3. UMP
4. GMP
5. CDP
6. ADP
7. UDP
8. CTP
9. GDP
10. ATP
11. UTP
12. GTP
Column : Shodex IEC DEAE-825 (8.0 mm I.D. x 75 mm)
Eluent : (A); 0.1 M NaH2PO4 aq./CH3CN=80/20
(B); 0.5 M NaH2PO4 aq./CH3CN=80/20
Linear gradient: 0 min to 60 min, (A) to (B)
Flow rate : 1.0 mL/min
Detector : UV(260 nm)
Column temp. : 25 ℃
試料名インデックス
- Adenosine diphosphate, ADP
- Adenosine triphosphate, ATP
- Adenylic acid, Adenosine monophosphate, AMP
- Cytidine 5'-diphosphate, 5'-CDP
- Cytidine triphosphate, CTP
- Cytidylic acid, Cytidine monophosphate, CMP
- Guanosine diphosphate, GDP
- Guanosine triphosphate, GTP
- Guanylic acid, Guanosine monophosphate, GMP
- Uridine diphosphate, UDP
- Uridine triphosphate, UTP
- Uridylic acid, Uridine monophosphate, UMP