The Chinese Pharmacopoeia 2025 Edition*, Part IV, the monograph for Lactose, analysis by high‑performance liquid chromatography specifies the use of a column packed with amino silica or amino polymer gel. HILICpak VG-50 4E column confirmed the requirement was met.

System suitability requirementResolution between lactose and sucrose: > 1.5

*The version at the time of the application acquisition.

Sample: 10 µL
5 mg/mL each in H₂O/CH₃CN=50/50

1. 1.Sucrose
2. 2.Lactose