The Chinese Pharmacopoeia 2025 Edition*, Part IV, the monograph for Polydextrose, the determination of glucose, sorbitol, and 1,6‑anhydro‑β‑D‑glucose by high‑performance liquid chromatography specifies the use of a column packed with strong cation exchange resin (calcium form) in which sulfonic acid groups are bonded to a cross linked styrene divinylbenzene copolymer (7.8 mm (I.D.) x 300 mm (length), particle size: 10 µm). SUGAR SC1011 column confirmed the requirements were met.

System suitability requirements

Elution order: Glucose, 1,6‑anhydro‑β‑D‑glucose, Sorbitol
Resolution between each of glucose, 1,6‑anhydro‑β‑D‑glucose, and sorbitol: ≥ 1.0
Relative standard deviation (RSD): ≤ 5.0%

*The version at the time of the application acquisition.

Sample: 20 µL

1. 1.Glucose 0.16 mg/mL
2. 2.1,6-Anhydro-D-Glucose 0.08 mg/mL
3. 3.Sorbitol 0.08 mg/mL