The Chinese Pharmacopoeia 2025 Edition*, Part IV, the high‑performance liquid chromatography method for “Trehalose” specifies the use of a column packed with styrene–divinylbenzene copolymer–based cation‑exchange resin in the sodium form (or hydrogen form). SUGAR KS-801 column confirmed the requirements were met.

System suitability requirements

Relative standard deviation (RSD) of the main peak area: ≤ 2.0%
Resolution between the trehalose peak and the adjacent preceding and following peaks: > 1.5

*The version at the time of the application acquisition.

Sample: 20 µL
(Standard solution) Diluted in H₂O

1. 1.Maltotriose 2.5 mg/mL
2. 2.Trehalose 10 mg/mL
3. 3.Glucose 2.5 mg/mL