National Standard of China GB 23200.122-2026*, "National food safety standard - Determination of four pesticides including glyphosate and their metabolites residues in foods of plant origin - Liquid chromatography-tandem mass spectrometry method", specifies the use of a quaternary ammonium-modified polyvinyl alcohol-based anion-exchange column or a column with equivalent performance. In this application, LC/MS/MS analyses of four phosphonic acid amino acid herbicides, including glyphosate and glufosinate, and six of their metabolites were performed using IC SI-50 4D.
Note: Although the GB method specifies the use of water for the preparation of N-methylglyphosate stock standard solution, the N-methylglyphosate reagent used in this application was insoluble in water and was therefore dissolved in 1 M NaOH aqueous solution. All other analytical conditions conformed to the GB method.

Preparation of standard solutions

1. 1.Dissolve N-methylglyphosate in 1 M NaOH aqueous solution and dissolve all other reagents in water to prepare individual stock standard solutions at 1000 mg/L.
2. 2.Mix the stock standard solutions and dilute with water to prepare a mixed intermediate standard solution at 50 mg/L.
3. 3.Dilute the mixed intermediate standard solution prepared in step 2 with water to prepare a mixed standard solution at 1 mg/L.

*The version at the time of the application acquisition.

Sample: 10 µL

(Sample solution) 1 µg/mL each
Phosphonic acid
AMPA; Aminomethylphosphonic acid
Ethephon
MPPA; 3-Methylphosphinicopropionic acid
N-Acetyl AMPA
Glyphosate
Glufosinate ammonium
N-Methyl glyphosate
N-Acetyl glyphosate
N-Acetyl glufosinate