The National Standards of China GB/T 20882.5-2025*, Quality requirements for starch sugar - Part 5: Maltose, lists two HPLC methods for the determination of maltose content.

*The version at the time of the application acquisition.

A column bonded with amino groups (4.6 mm (I.D.) x 250 mm (length), particle size: 5 µm) or an equivalent column is specified in the method. Analysis of a maltose standard using Asahipak NH2P-50 4E confirmed that the system suitability requirement was met. In addition, the calibration curve for maltose showed good linearity.

System Suitability RequirementDifference between two replicate measurements under repeatability conditions: ≤ 0.5% of the mean of the two measurements

Sample: 20 µL
(Standard solution) 2 mg/mL each in 50 % CH₃CN

1. 1.Fructose
2. 2.Glucose
3. 3.Maltose
4. 4.Maltotriose

A cation-exchange column modified with Ca ion (7.8 mm (I.D.) x 300 mm (length), particle size: 9 µm) or an equivalent column is specified in the method. Analysis of a maltose standard using SUGAR SC1011 confirmed that the system suitability requirement was met. In addition, the calibration curve for maltose showed good linearity.

System Suitability RequirementDifference between two replicate measurements under repeatability conditions: ≤ 0.5% of the mean of the two measurements

Sample: 20 µL
(Standard solution) 2 mg/mL each in H₂O

1. 1.Maltotriose
2. 2.Maltose
3. 3.Glucose
4. 4.Fructose