The National Standards of China GB/T 20882.1-2025*, Quality requirements for starch sugar - Part 1: Edible dextrose, lists two HPLC methods for the determination of glucose content.

*The version at the time of the application acquisition.

A cation-exchange column modified with sulfonic acid groups (7.8 mm (I.D.) x 300 mm (length), particle size: 9 µm) or an equivalent column, is specified in the standard. For an analysis of a glucose standard using SUGAR SH1011 confirmed the requirement was met. In addition, the calibration curve for glucose showed a good linearity.

System suitability requirement

Difference between two replicate measurements under repeatability conditions: ≤ 0.5% of the mean of the two measurements

Sample: 20 µL
Diluted in 0.05 % H₂SO₄ aq.

1. 1.Glucose 2 mg/mL

 

1.  

A cation-exchange column modified with Ca ion (7.8 mm (I.D.) x 300 mm (length), particle size: 9 µm) or an equivalent column, is specified in the standard. For an analysis of a glucose standard using SUGAR SC1011 confirmed the requirement was met. In addition, the calibration curve for glucose showed a good linearity.

System suitability requirement

Difference between two replicate measurements under repeatability conditions: ≤ 0.5% of the mean of the two measurements

Sample: 20 µL
Diluted in H₂O

1. 1.Glucose 2 mg/mL

 

1.