In accordance with the provisions of the “Food Safety Law of the People’s Republic of China,” D-allose has been approved as a new food ingredient. For the “Assay” test with liquid chromatography of D-allulose, the method specifies the use of a Ca type cation-exchange column (6.5 mm (I.D.) x 300 mm (length), particle size: 10 µm) or a column with equivalent performance. Using SUGAR SC1011 column confirmed the requirements were met. Also a good linear calibration curve for D-allulose was obtained.

System suitability requirementsDifference between two replicate measurements under repeatability conditions: ≤ 1% of the mean of the two measurements.

D-Allulose (sample prepared by dissolving 1 g and diluting to 100 mL)
Limit of detection (LOD): 0.1 g/100 g
Limit of quantification (LOQ): 0.3 g/100 g

*The version at the time of the application acquisition.

Sample: 10 µL

1. 1.Allulose 2 mg/mL

 

1.