The ratio of deproteinization with MSpak GF-4A (a column for Column switching) and with a corresponding column of other company were compared. Since the ratio of deproteinazation is high, GF-2A can suppress the adsorption of protein to analytical columns or LC/MS as much as possible, and keep the reproducibility of analysis. (GF-2A (2.0 mm I.D. x 10 mm) is a custom-made column of GF-4A.)
Sample: 200 mg/mL BSA 100 μL
|Ratio of Deproteinization|
Pretreatment Eluent : 10 mM CH3COONH4(pH7.0) Flow rate : 0.5 mL/min Column temp. : Room temp. Switching time : 5 min Analysis Column : Shodex PROTEIN *KW-604S (6.0 mm I.D. x 50 mm) *KW-604S is phase-out product. Eluent : 0.1 % TFA in (H2O/CH3CN=50/50) Flow rate : 0.5 mL/min Detector : UV (280 nm) Column temp. : Room temp.
Operation Manual / Certificate of Analysis
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