The ratio of deproteinization with MSpak GF-4A (a column for Column switching) and with a corresponding column of other company were compared. Since the ratio of deproteinazation is high, GF-2A can suppress the adsorption of protein to analytical columns or LC/MS as much as possible, and keep the reproducibility of analysis. (GF-2A (2.0 mm I.D. x 10 mm) is a custom-made column of GF-4A.)

Pretreatment
Eluent         : 10 mM CH3COONH4(pH7.0)
Flow rate      : 0.5 mL/min
Column temp.   : Room temp.
Switching time : 5 min
 
Analysis 
Column       : Shodex PROTEIN *KW-604S (6.0 mm I.D. x 50 mm) 
               *KW-604S is phase-out product.
Eluent       : 0.1 % TFA in (H2O/CH3CN=50/50)
Flow rate    : 0.5 mL/min
Detector     : UV (280 nm)
Column temp. : Room temp.