Ratio of Deproteinization

The ratio of deproteinization with MSpak GF-4A (a column for Column switching) and with a corresponding column of other company were compared. Since the ratio of deproteinazation is high, GF-2A can suppress the adsorption of protein to analytical columns or LC/MS as much as possible, and keep the reproducibility of analysis. (GF-2A (2.0 mm I.D. x 10 mm) is a custom-made column of GF-4A.)

Sample: 100 μL
BSA 200 mg/mL

Ratio of deproteinization
MSpak GF-2A Company A
99.80% 97.68%
Shodex MSpak GF-2A, Company A (2.0 mm I.D. x 50 mm each)
10 mM CH3COONH4 (pH7.0)
Flow rate
0.5 mL/min
Column temp.
Room temp.
Switching time
5 min


Shodex PROTEIN *KW-604S (6.0 mm I.D. x 50 mm)
*KW-604S is phase-out product.
0.1 % TFA in (H2O/CH3CN=50/50)
Flow rate
0.5 mL/min
UV (280 nm)
Column temp.
Room temp.

Operation Manual / Certificate of Analysis

Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

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Applications (Related Information)