LC/MS/MS Analysis of PFAS Based on the Korean Guideline for the Analysis of Prohibited Ingredients in Cosmetics (C18U 2B)

The Guideline for the Analysis of Prohibited Ingredients in Cosmetics (화장품 중 배합금지성분 분석법 가이드라인), published in Korea in 2024, includes analytical methods for PFAS compounds as well as tetraconazole, a fluorinated pesticide. In this application, six PFAS compounds that are frequently analyzed, together with tetraconazole, were analyzed using UHPLC ODS column C18U 2B. A polymer-based reversed-phase column, Asahipak ODP-50 4B, was used as a delay column* in this analysis method.
*Installing a delay column between the sample injector and the gradient mixer delays the elution of PFAS originating from the LC system to reduce its impact on PFAS quantification.

Sample: 5 µL
(Sample solution) 10 ng/mL each in CH3OH

  1. PFHxS; Perfluorohexanesulfonic acid
  2. PFOS; Perfluorooctanesulfonic acid
  3. PFOA; Perfluorooctanoic acid
  4. PFNA; Perfluorononanoic acid
  5. PFDA; Perfluorodecanoic acid
  6. PFDoA; Perfluorododecanoic acid
  7. Tetraconazole
Chromatograms of PFAS
Column
：Shodex C18U 2B (2.0 mm I.D. × 50 mm)
Delay column
：Shodex Asahipak ODP-50 4B (4.6 mm I.D. × 50 mm)
Eluent
：(A) 10 mM CH3COONH4 aq., (B) CH3CN
 
　(B %) 30 % (0 to 1 min), 30 to 80 % (1 to 11 min), 80 % (11 to 13 min), 80 to 30 % (13 to 14 min), 30 % (14 to 24 min)
Flow rate
：0.25 mL/min
Detector
：ESI-MS (MRM)
Column temp.
：40 ℃

Sample Name Index

Operation Manual / Certificate of Analysis

Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

Product Name Index

Applications