Comparisons of Two Separation Modes on PFAS LC/MS Analysis (C18U 2D, VT-50 2D)

PFAS stands for per- and polyfluoroalkyl substances. They are hardly decomposed in the environment, thus its persistence in the environment and accumulation in living organisms are concerned. In this application, PFAS with a terminal carboxyl group and alkyl chains of C4, C8, C10, and C12 were analyzed using a UHPLC compatible ODS column, C18U 2D, and a polymer-based HILIC mode column, HILICpak VT-50 2D. Their separation patterns were compared. Under a reverse phase mode (C18U 2D), longer-chain PFAS are retained longer. Whereas quaternary ammonium functional group modified on VT-50 2D packing material creates ion-exchange interactions, resulting in stronger retention for shorter-chain PFAS.

Please refer to application page "Analysis of PFAS (VT-50 2D)" and Shodex Technical Article No.22 "Analysis of PFAS Including Ultra Short-Chain PFAS.

Sample: 10 μL
50 ng/mL each  (in CH3OH/H2O=1/1)

  1. C12; PFDoA (Perfluorododecanoic acid)
  2. C10; PFDA (Perfluorodecanoic acid)
  3. C8; PFOA (Perfluorooctanoic acid)
  4. C4; PFBA (Perfluorobutanoic acid)
Chromatogram of PFAS_C18U 2D
Chromatogram of PFAS_VY-50 2D
Column
：Shodex C18U 2D (2.0 mm I.D. x 150 mm)
Eluent
：20 mM CH3COONH4 aq./CH3CN=50/50
Flow rate
：0.2 mL/min
Detector
：ESI-MS (SIM Negative)
Column temp.
：40 ℃
 
Column
：Shodex HILICpak VT-50 2D (2.0 mm I.D. x 150 mm)
Eluent
：50 mM CH3COONH4 aq./CH3CN=30/70
Flow rate
：0.2 mL/min
Detector
：ESI-MS (SIM Negative)
Column temp.
：40 ℃

 

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