Preparation Range of Agricultural Chemicals (EV2000AC-12F)

Gel permeation chromatography (GPC) is adopted in Shoku-An No. 1003001 (October 3rd, 2006, Japan) of the Pharmaceutical and Food Safety Bureau, MHLW, Section 2 “Simultaneous GC/MS (LC/MS) Analyses of Agricultural Chemicals in Livestock and Marine Products” for the purification process in the preparation method of testing solution. CLNpak EV-2000 AC corresponds to the above mentioned GPC column. In addition, CLNpak EV2000AC-12F corresponds to the GPC column mentioned in Shoku-An No. 0226 (February 26th, 2015, Japan) of the Pharmaceutical and Food Safety Bureau, MHLW, Section 2 “LC/MS Analyses of Agricultural Chemicals in Livestock and Marine Products”. Preparative range of agricultural chemicals is from the peak top of acrinathrin until the elution end of tricyclazole, but if purification is insufficient, it is documented that preparation range is preferably from the center of each retention time of acrinathrin and fluvalinate to the elution end of tricyclazole.


Sample : 2 mL
Acrinathrin
Tricyclazole
Fluvalinate


Columns      : Shodex CLNpak EV-G AC12C (12.0 mm I.D. x 100 mm)
               + EV2000AC-12F (12.0 mm I.D. x 300 mm)
Eluent       : Acetone/Cyclohexane=3/17
Flow rate    : 3 mL/min
Detector     : UV (254 nm)
Column temp. : 45 °C

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