The pigment removal rate for spinach pigment removal method was determined using CLNpak EV-2000 AC, a column for GPC clean-up. Most dyes elute in 40 - 60 mL.
Refer to "Spinach Pigment Removal (EV-2000 AC)".
Sample: 5 mL
- Corn oil
-
Spinach extract
Pigment removal rateScroll
| In 5 mL of spinach extract |
Amount of dye eluted in the given fraction range |
Total removal rate | ||
|---|---|---|---|---|
| Spinach (g) | Amount of pigment (dry weight, mg) |
40 - 60 mL | 60 - 130 mL | |
| 2.5 | 9.6 | 9.3 [97%] | 0.9 [9%] | 106% |
| 5 | 19.3 | 16.6 [86%] | 2.0 [10%] | 96% |
| 20 | 77.0 | 68.5 [89%] | 10.0 [13%] | 102% |
- Columns
- ：Shodex CLNpak EV-G AC (20 mm I.D. x 100 mm) + EV-2000 AC (20 mm I.D. x 300 mm)
- Eluent
- ：Acetone/Cyclohexane=20/80
- Flow rate
- ：5.0 mL/min
- Detector
- ：UV (254 nm)
- Column temp.
- ：25 ℃
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Elution Profile for Pesticides (EV-2000 AC)
- Effects of Eluent Components
- Separation with Acetone/Cyclohexane Eluent (EV-2000 AC)
- Separation with Ethyl Acetate/Cyclohexane Eluent (EV-2000)
- Spinach Pigment Removal (EV-2000 AC)
- Effects of Sample Injection Volume
- Effects of Flow Rate (EV-2000 AC)
- Effects of Column Temperature
- Preparation of Test Samples for Agricultural Chemicals in Livestock and Marine Products
- GPC Clean-up of Pesticide Residues (EV-2000 AC)
- Fractionation Standards (EV-2000 AC)
- Elution Volume for Lipid and Fatty Acids (EV-2000 AC)
- Effects of Temperature on GPC Clean-up
- Effects of Eluent Composition on GPC Clean-up
- GPC Clean-up Sample (1) (Fatty Tuna and Beef Tallow)
- GPC Clean-up Sample (2) (Milk and Egg)
- Preparation Range of Agricultural Chemicals (EV2000AC-12F)