An example of spinach pigment removal using CLNpak EV-2000 AC, a column for GPC clean-up, is shown. Most dyes elute in 40 - 60 mL.
Refer to "Spinach Pigment Removal - Removal Rate (EV-2000 AC)".
Sample: Spinach extract
- Column
- ：Shodex CLNpak EV-G AC (20 mm I.D. x 100 mm) + EV-2000 AC (20 mm I.D. x 300 mm)
- Eluent
- ：Acetone/Cyclohexane=20/80
- Flow rate
- ：5.0 mL/min
- Detector
- ：UV (254 nm)
- Column temp.
- ：25 ℃
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Sample Preparation for Animal and Fishery Products According to Japanese MHLW Notification
- GPC Clean-up for Animal and Fishery Products According to Japanese MHLW Notification (1) (EV-2000 AC)
- GPC Clean-up for Animal and Fishery Products According to Japanese MHLW Notification (2) (EV200AC-12F)
- Example of GPC Clean-up for Animal and Fishery Products According to Japanese MHLW Notification (1) (Medium Fatty Tuna and Beef Fat) (EV-2000 AC)
- Example of GPC Clean-up for Animal and Fishery Products According to Japanese MHLW Notification (2) (Milk, Egg) (EV-2000 AC)
- Elution Profile for Pesticides (EV-2000 AC)
- Effects of Temperature on GPC Clean-up
- Effects of Eluent Composition on GPC Clean-up
- Elution Volume for Lipid and Fatty Acids (EV-2000 AC)
- Effects of Eluent Components
- Separation with Acetone/Cyclohexane Eluent (EV-2000 AC)
- Separation with Ethyl Acetate/Cyclohexane Eluent (EV-2000)
- Spinach Pigment Removal - Removal Rate (EV-2000 AC)
- Effects of Sample Injection Volume
- Effects of Flow Rate (EV-2000 AC)
- Effects of Column Temperature
- Fractionation Standards (EV-2000 AC)