Instead of dichloromethane which may cause the environmental pollution, non-halogenated solvent such as ethyl acetate and acetone can be used as eluent. Each solvent is mixed with cyclohexane and used for CLNpak EV-2000 series as the eluent. The effect of the solvents and their mixing ratio is shown below.


Sample :
1. 125 mg/mL Corn oil
2. 1 mg/2 mL β-Carotene
3. 1 mg/2 mL Fluvalinate
4. 0.3 mg/2 mL Chinomethionate

Columns      : (Left); Shodex CLNpak EV-G AC (20 mm I.D. x 100 mm)
                       + EV-2000 AC (20 mm I.D. x 300 mm) 
               (Center); Shodex CLNpak EV-2000 (20 mm I.D. x 300 mm)
               (Right); Shodex CLNpak EV-G (20 mm I.D. x 100 mm)
                        + EV-2000 (20 mm I.D. x 300 mm)
Eluent       : (Left); Acetone/Cyclohexane
               (Center); Ethyl acetate/Cyclohexane
               (Right); Dichloromethane/Cyclohexane
Flow rate    : 4.0 mL/min
Detector     : UV (254 nm)
Column temp. : 25 °C