Two different temperature settings (room temerature and 40 °C) were compared for the GPC clean-up method using CLNpakEV-2000 AC . At room temperature, the high viscosity causes peak broadening and increases the analysis time. Therefore, analysis at 40 °C is preferred. In Food Sanitation Law #1003001 (Released by Ministry of Health, labour and welfare in Japan, 2006), analysis temperature is set at 40 °C.


Sample : 5 mL
Fatty tuna
Acrinathrin 40 mg/L
Tricyclazole 40 mg/L

Columns      : Shodex CLNpak EV-G AC (20.0 mm I.D. x 100mm)
               + EV-2000 AC (20.0 mm I.D. x 300 mm)
Eluent       : Acetone/Cyclohexane=1/4
Flow rate    : 5 mL/min
Detector     : UV (254 nm)
Column temp. : 40 °C or Rooom temp.