Elution volume for lipid and fatty acids were monitored by CLNpak EV-2000 AC. Lipid and fatty acids elute fast, thus by fractionating between acrinathrin and tricyclazol, most lipid and fatty acids can be removed. 
Sample : 5 mL
Beef tallow
Acrinathrin
Tricyclazole
Oleic acid
Palmitic acid
Linolenic acid
Linoleic acid
Columns : Shodex CLNpak EV-G AC (20.0 mm I.D. x 100 mm)
+ EV-2000 AC (20.0 mm I.D. x 300 mm)
Eluent : Acetone/Cyclohexane=1/4
Flow rate : 5 mL/min
Detector : UV (254 nm)
Column temp. : 40 °C
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Sample Preparation for Animal and Fishery Products According to Japanese MHLW Notification
- GPC Clean-up for Animal and Fishery Products According to Japanese MHLW Notification (1) (EV-2000 AC)
- GPC Clean-up for Animal and Fishery Products According to Japanese MHLW Notification (2) (EV200AC-12F)
- Example of GPC Clean-up for Animal and Fishery Products According to Japanese MHLW Notification (1) (Medium Fatty Tuna and Beef Fat) (EV-2000 AC)
- Example of GPC Clean-up for Animal and Fishery Products According to Japanese MHLW Notification (2) (Milk, Egg) (EV-2000 AC)
- Elution Profile for Pesticides (EV-2000 AC)
- Effects of Temperature on GPC Clean-up
- Effects of Eluent Composition on GPC Clean-up
- Effects of Eluent Components
- Separation with Acetone/Cyclohexane Eluent (EV-2000 AC)
- Separation with Ethyl Acetate/Cyclohexane Eluent (EV-2000)
- Spinach Pigment Removal (EV-2000 AC)
- Spinach Pigment Removal - Removal Rate (EV-2000 AC)
- Effects of Sample Injection Volume
- Effects of Flow Rate (EV-2000 AC)
- Effects of Column Temperature
- Fractionation Standards (EV-2000 AC)